Cleaning decontamination columns general guidelines flow cells general.
Waters hplc system cleaning procedure.
Residues or contaminations may block filters or capillaries.
10 nh4oh in water this removes peg and other.
Technical assistant 3 2 checking.
Water flush to clean acid from the system.
To clean the system.
Objective to lay down the procedure for operation of hplc waters alliance 2695 separation module scope to ensure that the instrument performs satisfactory and gives accurate and reproducible data.
Ion exchange to remove any remaining ions.
3 0 responsibility 3 1 doing.
Use solvent inlet filters to protect the system from incoming particles.
Controlling contamination in lc ms and hplc ms systems 715001307.
The water flushes are only needed to remove the previous solvent from the system.
Responsibility quality control officer executive.
Executive manager 4 0 accountability head of the department 5 0 procedure 5 1 for system cleaning.
The purification process should include all of the following steps.
Use only hplc grade solvents and water filtered through 0 2.
When preparing a system to run your method it is always important to prime up the system to ensure you get fresh solvents in all of the required lines.
Water acetonitrile methanol ipa 1 1 1 1 with 2 5 fa.
Place a b c and d seal wash and needle wash lines into a clean.
When working with any lc system setting it up and storing it is very important.
Water flush to clear customer s mobile phases from the system.
To clean the system.
For information on other acquity uplc cleaning methods refer to the recommended cleaning mixtures for lc table in the con trolling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
No injections are needed with that.
Disconnect the column and attach a union and flow directly to waste.
Flush the system using one of the cleaning mixtures as described in the cleaning solution table of the controlling contamination in uplc ms and hplc ms sytems guide 715001307.
2 0 scope this procedure is applicable for of all high performance liquid chromatography installed in quality control laboratory.
Remove all solvent filters.
It is necessary to restore the system flow path to a near new level of cleanliness note.
Maintaining your hplc uhplc uplc systems.
Cleaning mixtures for lc table in the controlling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
Label bottles correctly with bottle content and filling date expiry date.
Ultrapure water is water that has been purified through a system that targets contami nants detrimental to uplc ms and hplc ms systems.
